Our solution tackles a key challenge in traditional CRISPR experiments: the necessity for live cell cultures. By leveraging a cell-free transcription-translation (TXTL) system, we have crafted an in vitro environment optimal for CRISPR gene editing and regulation experiments. With the TXTL system, one can complete an experiment by simply combining various CRISPR components in a tube, eliminating many steps typical of conventional experiments.

Another hurdle has been experimental readouts and analysis. Traditional CRISPR gene knockouts and knockdowns often require quantification using costly equipment found only in labs. Our solution? We employ chromoproteins that produce vivid pigments easily seen by the naked eye. Furthermore, we have developed a novel computational algorithm CRISPectra capable of analyzing smartphone-captured images of these results, removing the need for specialized equipment.

Lowering Cost

Unlike traditional CRISPR experiments which cost thousands of dollars, our kits are approximately less than $5 each. We bypass the need for expensive lab equipment, making gene editing achievable anywhere, anytime.

Bridging Concept & Implementation

Biology education often suffers from a disconnect between theory and practical application. Our kits offer hands-on experience with CRISPR, facilitating a deeper understanding of the technology and its real-world application.